AP BIOLOGY

Detailed explanation-1: -DNA ligase works by forming two covalent phosphodiester bonds between the 3′ hydroxyl end of one nucleotide (acceptor) and the 5′ phosphate end of another (donor).

Detailed explanation-2: -DNA Ligase – Overview It is able to join the two DNA fragments as a result of the formation of a phosphodiester bond between them with the help of a molecule of energy. Cellular phenomena of recombination, DNA replication and repair generate breaks in the phosphate backbone DNA structure.

Detailed explanation-3: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.

Detailed explanation-4: -DNA ligases are enzymes required for the repair, replication and recombination of DNA. DNA ligases catalyse the formation of phosphodiester bonds at single-strand breaks in double-stranded DNA.