THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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rDNA (recombinant DNA)
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Bacteria cells
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Plasmid
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Detailed explanation-1: -A transgenic, or genetically modified, organism is one that has been altered through recombinant DNA technology, which involves either the combining of DNA from different genomes or the insertion of foreign DNA into a genome.
Detailed explanation-2: -Recombinant DNA technology is an extremely important research tool in biology. It allows scientists to manipulate DNA fragments in order to study them in the lab. It involves using a variety of laboratory methods to put a piece of DNA into a bacterial or yeast cell.
Detailed explanation-3: -Plasmids and bacteriophages are the most common vectors in recombinant DNA technology that are used as they have a very high copy number.
Detailed explanation-4: -DNA cloning In practice the procedure is carried out by inserting a DNA fragment into a small DNA molecule and then allowing this molecule to replicate inside a simple living cell such as a bacterium. The small replicating molecule is called a DNA vector (carrier).
Detailed explanation-5: -Recombinant DNA technology involves the selection of the desired gene for administration into the host followed by a selection of the perfect vector with which the gene has to be integrated and hence the recombinant DNA is formed. This recombinant DNA, then has to be introduced into the host.