THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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During screening, blue colonies is the bacteria that ____
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Carry non recombinant plasmid
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Carry recombinant plasmid
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No plasmid
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Died
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Explanation:
Detailed explanation-1: -Isopropyl -D-1-thiogalactopyranoside (IPTG) is used along with X-gal for blue-white screening.
Detailed explanation-2: -Blue colonies contain a plasmid, however they are producing ß-galactosidase (evident by their ability to convert X-GAL into a blue precipitate) indicating that they do not contain inserts.
Detailed explanation-3: -Reason: Presence of insert results in inactivation of enzyme beta-galactosidase known as insertional inactivation.
Detailed explanation-4: -Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
There is 1 question to complete.