AP BIOLOGY

Detailed explanation-1: -S1 nuclease (from Aspergillus oryzae) is a specific enzyme to degrade single stranded DNA or RNA molecules. It has been reported to be able to convert superhelical circular DNA molecules into open circle or linear forms under certain conditions, but this function has not been well explored.

Detailed explanation-2: -Nuclease S1 digests single-stranded regions of duplex DNA, RNA or DNA/RNA whereas nuclease P1 hydrolyses only single-stranded DNA and RNA.

Detailed explanation-3: -S1 Nuclease is supplied in a buffer of 10 mM sodium acetate (pH 4.6), 150 mM NaCl, 0.05 mM ZnSO4 and 50% glycerol.