AP BIOLOGY

Detailed explanation-1: -A polymerase ribozyme can be used to label the 3′ end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs.

Detailed explanation-2: -RNA polymerase (RNAP) is the essential enzyme responsible for transcribing the genetic information stored in DNA to RNA.

Detailed explanation-3: -Product Listing Application Overview. Detection of specific nucleic acid sequences can be accomplished by hybridization with a labeled RNA probe. RNA probes are sequences of variable lengths that are used to detect the presence of complementary nucleotide sequences in a sample.

Detailed explanation-4: -But the RNA polymerases of RNA viruses are the kings of errors – these enzymes screw up as often as one time for every 1, 000 – 100, 000 nucleotides polymerized. This high rate of mutation comes from the lack of proofreading ability in RNA polymerases. These enzymes make mistakes, but they can’t correct them.

Detailed explanation-5: -End-labeling is a rapid and sensitive method for radioactively, or nonisotopically, labeling DNA fragments and is useful in visualizing small amounts of DNA. End-labeling can also be used to label fragments at one end.