AP BIOLOGY

Detailed explanation-1: -Gene cloning, also known as molecular cloning, refers to the process of isolating a DNA sequence of interest for the purpose of making multiple copies of it. Classic gene cloning involves the following steps: Restriction enzyme digestion and ligation. Isolation of DNA.

Detailed explanation-2: -Gene cloning refers to the process by which a fragment of DNA is transferred from one organism to a vector. A vector is an agent that can carry a DNA fragment into a host cell. Commonly used vectors include plasmids, lambda phage, cosmid and yeast artificial chromosome (YAC).

Detailed explanation-3: -To get multiple copies of a gene or other piece of DNA you must isolate, or ‘cut’, the DNA from its source and then ‘paste’ it into a DNA vector that can replicate (or copy) itself.

Detailed explanation-4: -DNA cloning is the process of making multiple, identical copies of a particular piece of DNA. In a typical DNA cloning procedure, the gene or other DNA fragment of interest (perhaps a gene for a medically important human protein) is first inserted into a circular piece of DNA called a plasmid.

Detailed explanation-5: -To make a clone, scientists transfer the DNA from an animal’s somatic cell into an egg cell that has had its nucleus and DNA removed. The egg develops into an embryo that contains the same genes as the cell donor. Then the embryo is implanted into an adult female’s uterus to grow.