THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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coli with recombinant plasmid
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coli with Ca²+ ion
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coli with non-recombinant plasmid
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coli with no plasmid
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Detailed explanation-1: -Col plasmids, which contain genes that code for bacteriocins, proteins that can kill other bacteria. Degradative plasmids, which enable the digestion of unusual substances, e.g. toluene and salicylic acid. Virulence plasmids, which turn the bacterium into a pathogen. e.g. Ti plasmid in Agrobacterium tumefaciens.
Detailed explanation-2: -coli cell can only be transformed by one plasmid molecule, which is then propigated to make a clonal population of bacterial that contains only a single plasmid species. That is the common wisdom as I understand it. But is this true? And if so, how does it happen?
Detailed explanation-3: -Plasmids contain three components: an origin of replication, a polylinker to clone the gene of interest (called multiple cloning site where the restriction enzymes cleave), and an antibiotic resistance gene (selectable marker).