AP BIOLOGY

Detailed explanation-1: -How do restriction enzymes work? Like all enzymes, a restriction enzyme works by shape-to-shape matching. When it comes into contact with a DNA sequence with a shape that matches a part of the enzyme, called the recognition site, it wraps around the DNA and causes a break in both strands of the DNA molecule.

Detailed explanation-2: -restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule.

Detailed explanation-3: -Restriction enzymes can cut the strands of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand.

Detailed explanation-4: -Restriction Enzyme Types Generally, Type I enzymes cut DNA at locations distant to the recognition sequence; Type II cut DNA within or close to the recognition sequence; Type III cut DNA near recognition sequences; and Type IV cleave methylated DNA.

Detailed explanation-5: -A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.