THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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In the production of insulin cDNA, what is the step right after single-stranded cDNA produced?
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degradation of mRNA
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single-strand cDNA acts as template to produce second strand of cDNA
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ligation cDNA
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introduction of the rDNA
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Explanation:
Detailed explanation-1: -(i) Synthesis of gene (DNA) for human insulin artificially. (ii) Culturing recombinant E. coli in bio reactors. (iii) Insertion of human insulin gene into a plasmid.
Detailed explanation-2: -DNA directed synthesis of mRNA is called transcription.
Detailed explanation-3: -Insulin is synthesized as preproinsulin and processed to proinsulin. Proinsulin is then converted to insulin and C-peptide and stored in secretary granules awaiting release on demand. Insulin synthesis is regulated at both the transcriptional and translational level.
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