THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
Klenov fragment of DNA polymerase has which of following activities
|
|
5’ to 3’ polymerisation
|
|
|
5’ to 3’ exonuclease
|
|
|
3’ to 5’ exonuclease
|
Explanation:
Detailed explanation-1: -The Klenow fragment, which possesses 3′ to 5′ exo activity but lacks the 5′ to 3′ nick translation activity of Escherichia coli DNA polymerase I, catalyzes strand displacement synthesis as previously noted (9), but the distributive nature of this activity is evident in the presence of competitor DNA (Fig.
Detailed explanation-2: -DNA polymerase I is a single polypeptide chain with 928 amino acids and molecular weight of 109 kDa. It has three sites, which provide three distinct catalytic activities: 3′ to 5′ exonuclease, 5′ to 3′ exonuclease, and 5′ to 3′ polymerase.
Detailed explanation-3: -The polymerization and processivity rate is maximum in DNA polymerase III. It also has proofreading 3’
There is 1 question to complete.