THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Its action is specific
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Can be used outside of the cell
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Naturally present in most bacteria
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Prevent viral infection
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Detailed explanation-1: -What is the purpose of restriction enzymes in making recombinant DNA? Restriction enzymes cut the DNA into fragments at specific sites called restriction sites. These sites are then inserted into host DNA along with the restricted sites from vectors.
Detailed explanation-2: -Restriction enzymes have proved to be invaluable for the physical mapping of DNA. They offer unparalleled opportunities for diagnosing DNA sequence content and are used in fields as disparate as criminal forensics and basic research.
Detailed explanation-3: -No restriction enzyme is isolated from viruses.
Detailed explanation-4: -Recombinant DNA technology has also proven important to the production of vaccines and protein therapies such as human insulin, interferon and human growth hormone. It is also used to produce clotting factors for treating haemophilia and in the development of gene therapy.