THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What are the typical characteristics of a cloning vector?
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Bacterial cells cannot survive without it when grown under certain conditions.
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It contains restriction sites that allow the insertion of foreign DNA segments.
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It can replicate in bacterial cells.
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All of the above.
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Explanation:
Detailed explanation-1: -A cloning vector should possess an origin of replication so that it can self-replicate inside the host cell. It should have a restriction site for the insertion of the target DNA. It should have a selectable marker with an antibiotic resistance gene that facilitates screening of the recombinant organism.
Detailed explanation-2: -A cloning vector is a DNA molecule that has the following characteristics: (1) it is capable of replicating independently of the host chromosome; (2) an organism containing the vector can be grown preferentially; and (3) additional DNA can be inserted into the vector.
There is 1 question to complete.