THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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collection of total genomic DNA
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non-coding RNA molecule (containing about 22 nucleotides) found in plants,
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complementary DNA molecules synthesized from mRNA
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small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules
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Detailed explanation-1: -cDNA (short for copy DNA; also called complementary DNA) is synthetic DNA that has been transcribed from a specific mRNA through a reaction using the enzyme reverse transcriptase. While DNA is composed of both coding and non-coding sequences, cDNA contains only coding sequences.
Detailed explanation-2: -A cDNA probe can be generated from a specific mRNA. The mRNA, encoding a specific protein, is a template. By the action of reverse transcriptase and DNA polymerase, a cDNA is formed that can be used as a probe to hybridize with a specific gene sequence (Fig. 10.45).
Detailed explanation-3: -In molecular biology, complementary DNA (cDNA) is synthesised from an RNA template in a reaction catalysed by the enzyme reverse transcriptase (RTase).
Detailed explanation-4: -4.22 Reverse Transcription Polymerase Chain Reaction. Reverse transcription polymerase chain reaction (RT-PCR) is used to make a complementary DNA (cDNA) from the extracted mRNA for finding the specific protein-coding gene or for our cell to acquire the protein encoded in the mRNA.
Detailed explanation-5: -cDNA is often used to express a specific protein in a cell that does not normally express that protein (i.e., heterologous expression), or to sequence or quantify mRNA molecules using DNA based methods (qPCR, RNA-seq).