THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The DNA probe used to locate a particular gene in the genome.
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An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell.
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The laboratory apparatus used to clone genes.
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An enzyme that cuts DNA into restriction fragments.
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Detailed explanation-1: -In practice the procedure is carried out by inserting a DNA fragment into a small DNA molecule and then allowing this molecule to replicate inside a simple living cell such as a bacterium. The small replicating molecule is called a DNA vector (carrier).
Detailed explanation-2: -Cloning vectors are utilized to insert foreign DNA into another cell and create multiple copies of the same. The foreign DNA is duplicated and expressed utilizing the host cell machinery. It amplifies one copy of DNA into multiple copies.
Detailed explanation-3: -Scientists have taken advantage of plasmids to use them as tools to clone, transfer, and manipulate genes. Plasmids that are used experimentally for these purposes are called vectors. Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid.
Detailed explanation-4: -Cloning vector-a DNA molecule that carries foreign DNA into a host cell, replicates inside a bacterial (or yeast) cell and produces many copies of itself and the foreign DNA.
Detailed explanation-5: -Cloning vectors are tiny genomes into which foreign DNA can be introduced and transmitted to a host cell for use in cloning. Plasmids, bacteriophages, and cosmids are examples. A vector is a DNA molecule that carries a foreign DNA molecule into the host cell. It is self-replicating and can integrate into the host cell.