THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
To add new nucleotides to the growing strand of DNA.
|
|
|
To join nucleotides during replication.
|
|
|
To join nucleotides during transcription.
|
|
|
To cleave nucleic acids at specific sites.
|
Detailed explanation-1: -DNA restriction enzymes break DNA strands at specific sites based on the nucleic acid sequence. Thus, digestion with a given restriction enzyme or combination of restriction enzymes will produce fragments of different lengths that are directly related to the DNA sequence.
Detailed explanation-2: -To add new nucleotides to the growing strand of DNA.
Detailed explanation-3: -restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule.
Detailed explanation-4: -In the laboratory, restriction enzymes (or restriction endonucleases) are used to cut DNA into smaller fragments. The cuts are always made at specific nucleotide sequences. Different restriction enzymes recognise and cut different DNA sequences.
Detailed explanation-5: -Restriction endonucleases naturally target DNA duplexes. Systematic screening has identified a small minority of these enzymes that can also cleave RNA/DNA heteroduplexes and that may therefore be useful as tools for RNA biochemistry.