THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Because lacZ gene is distrupted/non functional
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because B-galactosidase is broken
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Because hydrolyze x-gal sugar is formed
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lac z gene is iintact
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Detailed explanation-1: --Galactosidase has three enzymatic activities (Fig. 1). First, it can cleave the disaccharide lactose to form glucose and galactose, which can then enter glycolysis. Second, the enzyme can catalyze the transgalactosylation of lactose to allolactose, and, third, the allolactose can be cleaved to the monosaccharides.
Detailed explanation-2: -The disorder is caused by mutations in the GLB1gene (3p22. 3) coding for beta-galactosidase. To date, more than 165 mutations have been identified. Deficient enzyme activity leads to toxic accumulation of gangliosides in body tissues, and particularly in the central nervous system (CNS).
Detailed explanation-3: -The lac Z gene codes for -galactosidase, the main enzyme of the lac operon as it catalyzes the hydrolysis of lactose by converting lactose into glucose and galactose.
Detailed explanation-4: -The enzyme beta-galactosidase is involved in a certain body reaction. What will most likely happen if beta-galactosidase is not available? The rate of the reaction will change.