THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium? I) Transform bacteria with recombinant DNA molecule.II) Cut the plasmid DNA using restriction enzymes.III) Extract plasmid DNA from bacterial cells.IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.V) Use ligase to seal plasmid DNA to non-plasmid DNA
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I, II, IV, III, V
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III, II, IV, V, I
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II, III, V, IV, I
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III, IV, V, I, II
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Explanation:
Detailed explanation-1: -(i) Transform bacteria with recombinant (ii) Cut the plasmid DNA using restriction enzyme . (iii) Extract plasmid DNA to-DNA fragments DNA (iv) Bind the plasmid DNA to non-plasmid DNA fragments .
Detailed explanation-2: -(i) Transformbacteria with recombinant DNA molecule. (ii) Cut the plasmid DNA using restriction enzymes. (iii) Extract plasmid DNA from bacterial cells. (iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
Detailed explanation-3: -The correct option is B I
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