AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which of following statements describes a restriction enzyme? (can be more than 1)
A
It seals the inserted gene of interest to an open plasmid
B
It hydrolyses the phosphodiester backbone of each strand
C
It recognises palindromic base sequences
D
It stimulates the replication of a recombinant DNA
Explanation: 

Detailed explanation-1: -Restriction Enzyme Recognition Site Many restriction enzymes or restriction endonucleases identify a specific palindrome site and cut the DNA strands. They mostly cut the DNA, slightly away from the centre of the palindrome and between the same two nitrogenous bases in both the strands.

Detailed explanation-2: -Restriction enzymes cut double-stranded DNA * at specific locations based the pattern of bases found at those locations. These enzymes predictably cut both strands because the sequences they recognize are palindromic.

Detailed explanation-3: -1 Answer. Because it is more efficient.

Detailed explanation-4: -Answer and Explanation: Restriction enzymes do not cut at regular intervals; instead, they cut the DNA anywhere they find their specific restriction site.

There is 1 question to complete.