THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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plasmid & genes are cut
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cut DNA joined with ligase to make rDNA plasmids
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plasmid & genes are cut
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cut DNA joined with ligase to make rDNA plasmids
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Detailed explanation-1: -Option 1 is the correct sequence. Plasmid and gene of interest are cut using restriction enzymes and ligated using DNA ligase. This becomes a rDNA. This is inserted into a bacterial host cell.
Detailed explanation-2: -(1) Isolate the DNA sequence gene of interest. (2) Insert your DNA of interest into the vector plasmid: (a) cut both vector DNA and gene of interest with the same restriction enzymes, (b) mix the DNAs: they join by base pairing, (c) add DNA ligase to bond the pieces covalently. The result is a recombinant plasmid.
Detailed explanation-3: -There are six steps involved in rDNA technology. These are – isolating genetic material, restriction enzyme digestion, using PCR for amplification, ligation of DNA molecules, Inserting the recombinant DNA into a host, and isolation of recombinant cells.
Detailed explanation-4: -Once a recombinant plasmid is made that contains a gene of interest, such as insulin, the plasmid can enter bacterial cells by a process called transformation.