THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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DNA ligase
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Restriction enzyme
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RNA polymerase
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DNA polymerase
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Detailed explanation-1: -So, the correct answer is ‘Ligase’
Detailed explanation-2: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA.
Detailed explanation-3: -Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA cloning to join restriction endonuclease fragments that have annealed. The ligase commonly used is T4 DNA ligase, which was first isolated from E. coli that were infected with the lytic bacteriophage T4.
Detailed explanation-4: -They are called sticky ends. DNA ligase is the enzyme which facilitates the sticky ends to join with eachother and forms Recombinant DNA. Was this answer helpful?
Detailed explanation-5: -Ligases can join any DNA fragments with ‘blunt’ ends. They can also join DNA fragments with ‘sticky’ ends, but only if the nucleotides on the strands are complementary. To get complementary ‘sticky ends’ the DNA fragments to be joined must be cut with the same restriction enzyme.