AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which of the following enzymes are NOT used in the production of a recombinant DNA?
A
DNA polymerase
B
DNA ligase
C
Restriction enzymes
D
Restriction endonuclease
Explanation: 

Detailed explanation-1: –The type-I recognizes specific sequences in the DNA of interest but at non-specific sites. It cuts at more than 1, 000 bp away from the gene of interest. Thus, it is incompatible to be used.

Detailed explanation-2: -E. coli is not required for preparation of a recombinant DNA molecule rather it may be required for the expression of recombinant DNA-molecule.

Detailed explanation-3: -DNA Ligase-It ligates the Vector DNA with the foreign DNA and forms the recombinant DNA.

Detailed explanation-4: -Detailed Solution. The correct answer is Bt-cotton. Bt-cotton is not a product of Recombinant DNA Technology.

There is 1 question to complete.