THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which of the following enzymes are used in gene technology?
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DNA polymerase
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DNA ligase
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Restriction enzyme
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RNA polymerase
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Explanation:
Detailed explanation-1: -Ligase is used to join the DNA fragments cut by the restriction enzymes.
Detailed explanation-2: -Genetic engineering became possible with the discovery of mainly two types of enzymes: the cutting enzymes called restriction endonucleases and the joining enzymes called ligases.
Detailed explanation-3: -Type II restriction enzymes are heavily used in recombinant DNA techniques. Type II enzymes consist of single, separate proteins for restriction and modification. One enzyme recognizes and cuts DNA, the other enzyme recognizes and methylates the DNA.
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