THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which of the following enzymes joins the paired sticky ends of DNA fragments?
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DNA ligase
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DNA polymerase
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reverse transcriptase
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restriction enzymes
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Explanation:
Detailed explanation-1: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA.
Detailed explanation-2: -Sticky ends can be joined by using DNA ligases. Each restriction enzyme functions by inspecting the length of a DNA sequence.
Detailed explanation-3: -DNA Ligase joins DNA fragments.
Detailed explanation-4: -To join sticky ends DNA ligase enzyme is used which acts by catalyzing the formation of a phosphodiester bond. DNA ligases are called Molecular glue since these enzymes are used during replication for joining phosphodiester backbone, used in RDT for joining sticky ends, and also used in damage and repair cells.
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