AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
A
restriction endonuclease cleaving gene of interest and plasmid.
B
production of recombinant DNA using DNA ligase and a mixture of cut gene of interest and plasmid.
C
transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin.
D
probing blots of bacteria clones with radioactive DNA complementary to the desired gene.
Explanation: 

Detailed explanation-1: -So, the correct answer is ‘Heat shock at 42oC42oC for 90 seconds’

Detailed explanation-2: -So, the correct option is ‘Karyotyping of the host DNA’.

There is 1 question to complete.