AP BIOLOGY

Detailed explanation-1: -As shown in Figure 1, the production of cDNA involves two basic steps: separating mRNA molecules from other cellular RNA, and then using reverse transcriptase to copy the mRNA molecules into cDNA.

Detailed explanation-2: -Perform cDNA synthesis Reverse transcription reactions involve three main steps: primer annealing, DNA polymerization, and enzyme deactivation. The temperature and duration of these steps vary by primer choice, target RNA, and reverse transcriptase used. The critical step is during DNA polymerization.

Detailed explanation-3: -The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA).

Detailed explanation-4: -In biology, the process in cells by which an enzyme makes a copy of DNA from RNA. The enzyme that makes the DNA copy is called reverse transcriptase and is found in retroviruses, such as the human immunodeficiency virus (HIV).

Detailed explanation-5: -By using reverse transcriptase, retro virus incorporate their genetic material into host’s genetic material. So, the correct answer is ‘Transcribes viral RNA to cDNA’.