THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The heat from surrounding increase denaturating process
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The medium is too acidic for the bacteria
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The bacteria does not have cloning vector with ampR
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Detailed explanation-1: -coli has acquired -lactamases, i.e. enzymes that hydrolyse the -lactam ring and thus inactivate ampicillin. A range of -lactamases are produced by Gram-negative bacteria, the most common -lactamases being TEM-1, TEM-2 and SHV-1, which confer resistance to ampicillin/amoxicillin and early generation cephalosporins.
Detailed explanation-2: -The bacterium cannot grow in the presence of the antibiotic ampicillin unless it contains the plasmid, and so there will be no growth on the LB/Amp plate of the bacteria without the plasmid.
Detailed explanation-3: -Answer and Explanation: To be useful, a plasmid-derived cloning vector should replicate easily from its own origin of replication, produce many copies of itself per bacterium, have a multiple cloning site (polylinker), and harbor a selectable marker.
Detailed explanation-4: -Ampicillin (AMP), a semi-synthetic -lactam antibiotics, is widely used to treat of human and livestock E. coli infection, but recently its resistance rate has increased. AMP works on the active replicating stage of bacteria, inhibiting the synthesis of bacterial cell wall.