THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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mRNA doesn’t contain intron
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mRNA consist code for specific target gene
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Difficult to find one gene among all the genes in the nucleus
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mRNA is easy to isolate
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Detailed explanation-1: -Expert-Verified Answer. DNA has introns because splicing has not occurred, but mRNA just has the coding DNA, meaning that the entire molecule will code for insulin.
Detailed explanation-2: -Like its name sounds, this machine makes DNA. Luckily the insulin gene is small since these machines could only make small snippets of DNA. A second group managed to fish it out of human DNA. This was done by putting random pieces of human DNA into bacteria and finding the bacterium that had the insulin gene.
Detailed explanation-3: -The human insulin gene is removed using a restriction enzyme . A bacterial plasmid is cut open using the same restriction enzyme . Restriction enzymes leave ‘sticky ends’, where one of the two DNA strands is longer than the other.