THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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To cut the DNA molecules at a specific restriction site.
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To join the gene of interest with the enzyme.
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To produce a complementary sticky end or blunt end.
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To produce specific base sequences of DNA.
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Detailed explanation-1: -By using the same enzyme to cut the bacterial plasmid where the gene of interest needs to be inserted, complementary sticky ends will be created at the extremities of the plasmid. Due to the complementarity of these sticky ends, the two DNA fragments can stick to one another.
Detailed explanation-2: -The restriction enzymes cuts at fixed sequences, therefore same restriction enzyme must be used since it forms fragments with complementary sticky ends, therefore facilitating the formation of bonds between them.
Detailed explanation-3: -Explanation: Restriction enzymes cut at specific sequences so the same restriction enzyme must be used because it will produce fragments with the same complementary sticky ends, making it possible for bonds to form between them. There are certain compatible restriction sites that can be used together.
Detailed explanation-4: -Using two different restriction enzyme sites can help ensure the correct orientation of the gene of interest when it is inserted and prevent the plasmid vector from ligating with itself.
Detailed explanation-5: -Sticky ends are helpful in cloning because they hold two pieces of DNA together so they can be linked by DNA ligase.