AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

THE HUMAN GENOME

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
a technique used to separate nucleic acids or proteins according to size and charge in an agarose or acrylamide gel
A
genetic engineering
B
vector
C
gel electrophoresis
D
two-dimensional gel electrophoresis
Explanation: 

Detailed explanation-1: -Electrophoresis is a technique that enables separation and analysis of charged molecules in an electric field. Gel electrophoresis is most commonly used for separation and purification of proteins and nucleic acids that differ in size, charge, or conformation. The gel is composed of polyacrylamide or agarose.

Detailed explanation-2: -Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.

Detailed explanation-3: -Nucleic acid extraction consists of three major processes: isolation, purification, and concentration. Commercial extraction kits are commonly used in the clinical microbiology laboratory [2]. These kits provide the essential requirements for nucleic acid extraction.

Detailed explanation-4: -Electrophoresis Electrophoresis is a laboratory technique used to separate DNA, RNA or protein molecules based on their size and electrical charge. An electric current is used to move the molecules through a gel or other matrix.

Detailed explanation-5: -In this technique, DNA and RNA molecules are separated by applying an electric field to move negatively charged nucleic acids through an agarose or polyacrylamide gel matrix. The gel matrix acts as a sieve, allowing shorter molecules to run more quickly through the pores of the gel than longer molecules.

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