AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

THE HUMAN GENOME

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
In order to insert a human gene into plasmid, both must
A
code for the same gene product.
B
be cut by the same restriction enzyme.
C
originate from the same type of cell.
D
have identical sequences.
Explanation: 

Detailed explanation-1: -The restriction enzymes cuts at fixed sequences, therefore same restriction enzyme must be used since it forms fragments with complementary sticky ends, therefore facilitating the formation of bonds between them.

Detailed explanation-2: -The same restriction enzyme should be used when cutting out a gene of interest and cutting the plasmid vector where the DNA will be inserted so that the sticky ends of both will match up.

Detailed explanation-3: -So, the correct answer is ‘Ligase. ‘

There is 1 question to complete.