THE MOLECULAR BASIS OF INHERITANCE
THE HUMAN GENOME
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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cut the DNA into fragments
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transfer the DNA to another source
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clone the DNA needed
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add the DNA into the wells in the electrophoresis machine
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Detailed explanation-1: -Restriction enzymes, also called restriction endonucleases, recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. They are indispensable to the isolation of genes and the construction of cloned DNA molecules.
Detailed explanation-2: -How do restriction enzymes work? Like all enzymes, a restriction enzyme works by shape-to-shape matching. When it comes into contact with a DNA sequence with a shape that matches a part of the enzyme, called the recognition site, it wraps around the DNA and causes a break in both strands of the DNA molecule.
Detailed explanation-3: -Definition. A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.
Detailed explanation-4: -Restriction enzymes, found naturally in bacteria, can be used to cut DNA fragments at specific sequences, while another enzyme, DNA ligase, can attach or rejoin DNA fragments with complementary ends.