THE MOLECULAR BASIS OF INHERITANCE
THE HUMAN GENOME
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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PCR would proceed normally
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Non-specific PCR of random templates will occur
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The reaction will cease after a few cycles
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The PCR reaction will not commence
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Detailed explanation-1: -The PCR requires primers, dNTPs, and Taq DNA Polymerase. Without these, PCR will not commence. DNA denaturation would occur, but there would be no annealing and renaturation steps without primers, dNTPs, and Taq polymerase.
Detailed explanation-2: -What would be the effect on a typical PCR reaction if there are no dNTPs in the reaction? PCR would proceed normally. The reaction will cease after a few cycles.
Detailed explanation-3: -Taq polymerase enzyme adds the new deoxyribonucleotide bases to the 3’ end of the primer. If primer is not present, then Taq polymerase will have no place to add nucleotides and carry out amplification. Therefore, DNA elongation will not take place.
Detailed explanation-4: -Answer and Explanation: Taq polymerase is the enzyme which helps in the formation of the daughter DNA strand. It polymerises the new strand on the exposed template by adding nucleotides. In the absence of the Taq polymerase, the elongation step will not occur and there will not be any net amplification.