THE MOLECULAR BASIS OF INHERITANCE
TOOLS AND TECHNIQUES OF RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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True
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False
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Detailed explanation-1: -DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode. Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.
Detailed explanation-2: -Supercoiled plasmid DNA, because of its compact conformation, moves through the gel fastest, followed by a linear DNA fragment of the same size, with the open circular form traveling the slowest.
Detailed explanation-3: -DNA fragments are negatively charged, so they move towards the positive electrode. Because all DNA fragments have the same amount of charge per mass, small fragments move through the gel faster than large ones.
Detailed explanation-4: -Negatively charged DNA/RNA migrates through the pores of an agarose gel towards the positively charged end of the gel when an electrical current is applied, with smaller fragments migrating faster.
Detailed explanation-5: -Agarose Gel Electrophoresis Lower molecular weight (lower length) DNAs will move faster through gel matrix pores than larger ones. However, the shape of a DNA molecule also plays a role in its movement, with the fastest moving form known as “supercoiled” DNA.