AP BIOLOGY

Detailed explanation-1: -A simpler approach is PCR/RFLP analysis in which PCR amplification of a specific gene is followed by restriction enzyme digestion.

Detailed explanation-2: -RFLP, as a molecular marker, is specific to a single clone/restriction enzyme combination. Most RFLP markers are co-dominant (both alleles in heterozygous sample will be detected) and highly locus-specific.

Detailed explanation-3: -Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) has been used successfully to detect SNPs linked to resistance, and it is a sensitive, specific, low cost and easy to apply technique. Moreover, the process of restriction enzyme digestion yields a quick result by gel analysis.

Detailed explanation-4: -The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method is based on the digestion of PCR amplicons with appropriate restriction enzymes to produce distinct polymorphic fragments used as markers for species identification [7, 8].

Detailed explanation-5: -Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA.