AP BIOLOGY

Detailed explanation-1: -element restricts the transposon and the target DNA in a combination of which of the following? (A) The target DNA of transposase restriction mechanism is a combination blunt end cut for transposon and sticky end cut for target DNA.

Detailed explanation-2: -DNA transposases are enyzmes that move discrete segments of DNA called transposons from one location in the genome (often called the donor site) to a new site without using RNA intermediates. DNA transposases are usually encoded by the mobile element itself (in which case they are “autonomous” transposons).

Detailed explanation-3: -The process starts when two copies of enzyme bind to the DNA at the two ends of the transposon. Then, the two ends are brought together, closing the transposon into a big loop, and the transposase cuts the DNA at both ends.

Detailed explanation-4: -A transposase is any of a class of enzymes capable of binding to the end of a transposon and catalysing its movement to another part of a genome, typically by a cut-and-paste mechanism or a replicative mechanism, in a process known as transposition.

Detailed explanation-5: -There are two essential features for DNA-based transposons. The first is that they contain inverted repeats at either end. The second is that they have a gene which encodes transposase. Transposase is an enzyme that recognizes the inverted repeats and moves the DNA contained within those repeats to a new location.