AP BIOLOGY

Detailed explanation-1: -All normal tissue-derived cells (except those derived from the haematopoietic system) are anchorage-dependent cells and need a surface/cell culture support for normal proliferation.

Detailed explanation-2: -So what is anchorage dependence? The majority of cells must attach to a surface, matrix, or substratum to survive, grow, and divide. These types of cells are referred to as anchorage dependent cells. These cells can either be in the body or cultured/grown in laboratory-controlled conditions.

Detailed explanation-3: -Definition. Anchorage dependence of survival, growth etc. describes the need for cells to attach to a solid substrate in order to exert the activities indicated. Anchorage independence describes the property of transformed cells to form aggregates/colonies in semi-solid agar medium without adherence to the substrate.

Detailed explanation-4: - These cells form a primary culture that usually attach onto the glass surface of flask in monolayer form. The cells growing on support surfaces are known as anchorage-dependent cells. Some cells grown in suspension culture and are known to be non anchorage-dependent cells.

Detailed explanation-5: -Cells anchor to dish surface and divide (anchorage dependence). When cells have formed a complete single layer, they stop dividing (density-dependent inhibition). If some cells are scraped away, the remaining cells divide to fill the gap and then stop (density-dependent inhibition).