AP BIOLOGY

Detailed explanation-1: -Enzymes will work best if there is plenty of substrate. As the concentration of the substrate increases, so does the rate of enzyme activity.

Detailed explanation-2: -Substrate concentration You could say that enzyme concentration has become the limiting factor. This is why a graph of substrate concentration vs rate of reaction plateaus (levels off) at the saturation point – all the active sites are saturated with substrate.

Detailed explanation-3: -Substrate concentration: Increasing substrate concentration also increases the rate of reaction to a certain point. Once all of the enzymes have bound, any substrate increase will have no effect on the rate of reaction, as the available enzymes will be saturated and working at their maximum rate.

Detailed explanation-4: -Increasing the enzyme concentration beyond a certain point does not change the rate of reaction BECAUSE when there are less substrate than enzyme: limiting factor is Substrate concentration.