AP BIOLOGY

Detailed explanation-1: -luteum. Colchicines prevent spindle formation during mitotic metaphase. Thus, it prevents the separation of chromosomes during anaphase stage.

Detailed explanation-2: -The microtubule cytoskeleton plays a crucial role in the cell cycle and in mitosis. Colchicine is a microtubule-depolymerizing agent that has long been used to induce chromosome individualization in cells arrested at metaphase and also in the induction of polyploid plants.

Detailed explanation-3: -T h e arrest of mitosis at metaphase The most striking effect of cold and of colchicine is the arrest of mitosis when the chromosomes have reached metaphase spiralization. This arrest leads to an accumulation of cells at this stage.

Detailed explanation-4: -Solution : Colchicine is obtained form cochicum autumnale plant, which inhibits the formation of spindle fibers in metaphase.

Detailed explanation-5: -Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing.