AP BIOLOGY

Detailed explanation-1: -The movement of a solute through a semi permeable membrane is called dialysis (as well as diffusion). The size of the minute pores in the dialysis tubing determines which substance can pass through the membrane. A solution of glucose and starch will be placed inside a bag of dialysis tubing.

Detailed explanation-2: -The dialysis membrane is one of the critical components that determine dialysis performance. These membranes allow only low-molecular-weight molecules, such as sodium, potassium, urea, and creatinine, to pass through while blocking proteins, such as albumin, and other larger molecules.

Detailed explanation-3: -Normal molecular weight cut-off is 12, 000 to 16, 000, and pore size is approximately 25 Angstroms. Tubing can be boiled or autoclaved and has a pH stability range of 5 to 9.

Detailed explanation-4: -It is important to note that every molecule is different; the concentration, interactions, and hydrophobicity of molecules can influence their ability to diffuse through a dialysis membrane. The temperature, volume, agitation rate and frequency of exchange of the external buffer are also important factors.

Detailed explanation-5: -Microscopic holes, or pores, in the dialysis tubing allow substances to be separated on the basis of their size. Molecules smaller than the pores pass freely across the tubing while larger molecules are trapped inside (or outside).