AP BIOLOGY

Detailed explanation-1: -Recall that DNA is replicated during the S phase of the cell cycle. Thus during metaphase of mitosis, each chromosome (i.e., each chromatid pair) will contain two molecules of double stranded DNA (one molecule per sister chromatid).

Detailed explanation-2: -During metaphase, the kinetochore microtubules pull the sister chromatids back and forth until they align along the equator of the cell, called the equatorial plane.

Detailed explanation-3: -The duplicated chromosomes then attach to the spindle, align at the cell equator, and move apart as the spindle microtubules retreat toward opposite poles of the cell. Each set of chromosomes is then surrounded by a nuclear membrane, and the parent cell splits into two complete daughter cells.

Detailed explanation-4: -Metaphase: During metaphase, each of the 46 chromosomes line up along the center of the cell at the metaphase plate.