LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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binds to the transcription terminator pause site
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function by wrapping nucleic acid around the single cleft
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essential transcription protein in prokaryotes
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unwinding the RNA transcript from DNA template in elongation step
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Detailed explanation-1: -Rho factor mediates termination of transcription by first binding to a site on the nascent transcript, then using its ATP hydrolysis activity as a source of energy to dissociate the transcript from its ternary complex with RNA polymerase and DNA.
Detailed explanation-2: -A factor (Rho factor) is a bacterial protein involved in the termination of transcription. Rho factor binds to the transcription terminator pause site, an exposed region of single stranded RNA (a stretch of 72 nucleotides) after the open reading frame at C-rich/G-poor sequences that lack obvious secondary structure.
Detailed explanation-3: -Rho is a hexameric helicase that binds RNA and translocates in the 5′-to-3′ direction using the energy derived from ATP hydrolysis. Initially, RNA is anchored to Rho primary binding sites on the surface of the hexamer. Rho recruitment sites tend to be rich in Cs and Us and free of strong secondary structures (6–8).