AP BIOLOGY

Detailed explanation-1: -In molecular biology, complementary DNA (cDNA) is synthesised from an RNA template in a reaction catalysed by the enzyme reverse transcriptase (RTase). cDNA synthesis is the first step in many molecular biology workflows, such as gene expression studies using real-time PCR.

Detailed explanation-2: -Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase, a DNA polymerase that can use either DNA or RNA as a template.

Detailed explanation-3: -In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA (miRNA)) template in a reaction catalyzed by the enzyme reverse transcriptase.

Detailed explanation-4: -Complementary DNA (cDNA) is synthesized in a reverse transcription reaction by the enzyme reverse transcriptase from a single-stranded mRNA template.

Detailed explanation-5: -By definition, cDNA is double-stranded DNA that was derived from mRNA which can be obtained from prokaryotes or eukaryotes. Once the mRNA is isolated, you need a few more reagents: dNTPs (dGTP, dCTP, dATP and dTTP), primers, and reverse transcriptase which is a DNA polymerase (figure 2).