LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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progresses away from the replication fork.
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occurs in the 3’ to 5’ direction.
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produces Okazaki fragments
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depends on the action of DNA polymerase
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Detailed explanation-1: -The replication of DNA occurs from 5’ to 3’ end on both strands of DNA i.e, leading strand and lagging strand. During the elongation of DNA, incoming DNA is attached to 3’ end of the elongated DNA which is catalyzed by DNA polymerase III.
Detailed explanation-2: -Answer and Explanation: The newly formed DNA strand is formed in a complementary fashion during elongation according to the template or mother strand. The leading strand adds up in the 5 prime to 3 prime direction complementarily with the help of DNA polymerase adding new nucleotides in the 3’ direction.
Detailed explanation-3: -DNA polymerase I extension unwinds the DNA, exposing a primosome assembly signal (n′ pas) or single-strand initiation A (ssiA) sequence on the leading strand (5). This single-stranded sequence motif allows assembly of the primosome through recruitment and activation of the PriA protein.
Detailed explanation-4: -During elongation, RNA polymerase “walks” along one strand of DNA, known as the template strand, in the 3’ to 5’ direction. For each nucleotide in the template, RNA polymerase adds a matching (complementary) RNA nucleotide to the 3’ end of the RNA strand.