LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Synthesis of cDNA molecule with a base complementary to DNA
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Synthesis of DNA molecule with a base complementary to DNA
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Synthesis of RNA molecule with a base complementary to DNA
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Synthesis of protein molecular with a base complementary to RNA
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Detailed explanation-1: -DNA transcription produces a single-stranded RNA molecule that is complementary to one strand of DNA. Transcription, however, differs from DNA replication in several crucial ways. Unlike a newly formed DNA strand, the RNA strand does not remain hydrogen-bonded to the DNA template strand.
Detailed explanation-2: -Transcription is the process by which the information in a strand of DNA is copied into a new molecule of messenger RNA (mRNA).
Detailed explanation-3: -Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase, a DNA polymerase that can use either DNA or RNA as a template.
Detailed explanation-4: -The process of synthesizing RNA from the genetic information encoded by DNA is called transcription. The enzymes involved in transcription are called RNA polymerases.
Detailed explanation-5: -Figure 2: RNA polymerase (green) synthesizes a strand of RNA that is complementary to the DNA template strand below it. Once RNA polymerase and its related transcription factors are in place, the single-stranded DNA is exposed and ready for transcription.