LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Saccharomyces
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Rhizobium
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Mycobacterium
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Escherichia
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Detailed explanation-1: -The development in the field of genetic engineering allowed the production of insulin in E. coli and yeast, which have been approved for therapeutic applications in human by FDA [14, 15]. Nowadays, recombinant human insulin is mainly produced either in E. coli or Saccharomyces cerevisiae.
Detailed explanation-2: -Nowadays, recombinant human insulin is mainly produced either in E. coli or Saccharomyces cerevisiae. Using E. coli expression system, the insulin precursors (IP) are produced as inclusion bodies and fully functional polypeptides are obtained finally by solubilization and refolding procedures [16].
Detailed explanation-3: -Detailed Solution The correct answer is Escherichia.
Detailed explanation-4: -coli by recombinant DNA technology. Synthetic genes for human insulin A and B chains are cloned separately in plasmid pBR322. The cloned synthetic genes are then fused to an Escherichia coli -galactosidase gene to provide efficient transcription and translation and a stable precursor protein.