LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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5’ cap
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chromatin packaging
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3’ Poly A tail
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splicing
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Detailed explanation-1: -Which of the following is NOT the step of mRNA processing? Explanation: mRNA processing occurs only in eukaryotes, it involves 5’capping, slicing of introns, polyadenylation, and RNA editing before transported to the cytoplasm, where they are translated to the ribosome.
Detailed explanation-2: -Three major events constitute pre-mRNA processing: (a) 5′-end capping, (b) splicing, and (c) 3′-end polyadenylation.
Detailed explanation-3: -The structure of a mature eukaryotic mRNA. A fully processed mRNA includes a 5’ cap, 5’ UTR, coding region, 3’ UTR, and poly(A) tail.
Detailed explanation-4: -mRNAs have three parts: Nucleotides at the 5′ end provide binding sites for proteins that initiate polypeptide synthesis; nucleotides in the middle specify the sequence of amino acids in the polypeptide; and nucleotides at the 3′ end regulate the stability of the mRNA (Fig. 1.1).