THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
Both require DNA polymerase.
|
|
|
Both require primers.
|
|
|
Both require deoxynucleotides (dNTPs).
|
|
|
Both require dideoxynucleotides (dNTPs).
|
|
|
Both generate new DNA products of equal length.
|
Detailed explanation-1: -Sanger sequencing is a DNA sequencing method in which target DNA is denatured and annealed to an oligonucleotide primer, which is then extended by DNA polymerase using a mixture of deoxynucleotide triphosphates (normal dNTPs) and chain-terminating dideoxynucleotide triphosphates (ddNTPs).
Detailed explanation-2: -Sanger sequencing differs from PCR in that only a single primer is used in the reaction. Typically, for a given PCR fragment, two Sanger sequencing reactions are set up, one for sequencing the forward strand, the other one for sequencing the reverse strand.
Detailed explanation-3: -Sanger sequencing requires a DNA template, a sequencing primer, a thermostable DNA polymerase, nucleotides (dNTPs), dideoxynucleotides (ddNTPs), and buffer. Thermal cycling in the sequencing reactions amplifies extension products that are terminated by one of the four ddNTPs.
Detailed explanation-4: -The dideoxynucleotides, or ddNTPSs, differ from the deoxynucleotides by the lack of a free 3′ OH group on the five-carbon sugar. If a ddNTP is added to a growing a DNA strand, the chain is not extended any further because the free 3′ OH group needed to add another nucleotide is not available.