THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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DNA Ligase
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Restriction enzyme
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Protease
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Carbohydrase
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Detailed explanation-1: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.
Detailed explanation-2: -DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.
Detailed explanation-3: -DNA ligases play an essential role in maintaining genomic integrity by joining breaks in the phosphodiester backbone of DNA that occur during replication and recombination, and as a consequence of DNA damage and its repair.
Detailed explanation-4: -ligase, also called Synthetase, any one of a class of about 50 enzymes that catalyze reactions involving the conservation of chemical energy and provide a couple between energy-demanding synthetic processes and energy-yielding breakdown reactions.