THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
Differentiate between transformant and non transformant
|
|
|
Differentiate between clones with vector plasmids and without vector plasmids
|
|
|
Differentiate between competent cells and non competent cells
|
|
|
Differntiate between negative and false negative
|
Detailed explanation-1: -The bacterial cells that take up the foreign DNA are called transformants, while the bacterial cells that do not take foreign DNA are called non-transformants. The transformants are distinguished from non-transformants with the help of antibiotic-resistant genes in the cloning vector.
Detailed explanation-2: -The lacZ gene encodes beta-galactosidase, which catalyzes the cleavage of lactose to form galactose and glucose.
Detailed explanation-3: -Transformants are cells that are introduced with the recombinant DNA molecule. Recombinants are carrier molecules that have foreign DNA inserted into their genome. They are cells that are able to express recombinant DNA. They are able to self replicate within the host organism.
Detailed explanation-4: -A scientist used a plasmid that contained ampicillin resistance gene and lacZ gene as selectable markers. He inserted the gene of interest within the ampicillin resistance gene instead of lacZ gene due to human error. The competent cells were allowed to take up the plasmid.