AP BIOLOGY

Detailed explanation-1: -A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.

Detailed explanation-2: -A restriction enzyme is a DNA-cutting enzyme that recognizes specific sites in DNA. Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang. If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase.

Detailed explanation-3: -Restriction endonucleases (REs) are bacterial enzymes that cleave double-stranded DNA.

Detailed explanation-4: -Abstract. Restriction endonucleases naturally target DNA duplexes. Systematic screening has identified a small minority of these enzymes that can also cleave RNA/DNA heteroduplexes and that may therefore be useful as tools for RNA biochemistry.

Detailed explanation-5: -Restriction endonucleases are named from the fact that they stop bacteriophages from multiplying by recognizing and cutting DNA at specified locations. Every endonuclease looks for the palindromic recognition sequence throughout the complete DNA sequence.